ABSTRACT
The aim of this study was to develop and validate an oligonucleotide suspension array for rapid identification of 15 bacterial species responsible for bacteremia, particularly prevalent in Chinese hospitals. The multiplexed array, based on the QIAGEN LiquiChip Workstation, included 15 oligonucleotide probes which were covalently bound to different bead sets. PCR amplicons of a variable region of the bacterial 23S rRNA genes were hybridized to the bead-bound probes. Thirty-eight strains belonging to 15 species were correctly identified on the basis of their corresponding species-specific hybridization profiles. The results show that the suspension array, in a single assay, can differentiate isolates over a wide range of strains and species, and suggest the potential utility of suspension array system to clinical laboratory diagnosis.
Subject(s)
Bacteremia , Diagnosis , Genetics , Bacterial Typing Techniques , Bacteriological Techniques , DNA Probes , Genetic Techniques , Listeria monocytogenes , Metabolism , Nucleic Acid Hybridization , Oligonucleotide Array Sequence Analysis , Oligonucleotides , Chemistry , RNA, Ribosomal , Chemistry , RNA, Ribosomal, 23S , Genetics , Stem CellsABSTRACT
<p><b>OBJECTIVE</b>To develop a high-throughput diagnostic method with suspension array technique for detecting pathogenic microbes.</p><p><b>METHODS</b>The probes and positive controls of 56 kinds of pathogenic microbes were designed, synthesized, and used to detect pathogenic microbes with suspension array technique.</p><p><b>RESULTS</b>Fluorescence signals of 56 positive controls were higher than those of the negative controls, and there was no cross-reaction between the probes and positive controls of different microbes.</p><p><b>CONCLUSION</b>Based on suspension array technique, the high-throughput diagnostic method may be useful in clinical detection of pathogenic microbes.</p>
Subject(s)
Animals , Humans , Communicable Diseases , Microbiology , Virology , Enterovirus , Microbiological Techniques , Mumps virus , Oligonucleotide Array Sequence Analysis , Methods , Pseudomonas aeruginosaABSTRACT
<p><b>OBJECTIVE</b>To observe human papillomavirus (HPV) infection in women with cervical lesions in Huzhou area of Zhejiang province.</p><p><b>METHODS</b>720 samples of cervical secretion or exfoliated cells were collected from women with cervical lesion in Huzhou area. Human papillomavirus was detected by suspension array technique.</p><p><b>RESULTS</b>Positive HPV infection was detected in 25.42% cases (183/720), with 135 cases of single HPV type, 33 of dual HPV types and 15 of multiple HPV types. HPV16 and HPV58 were the most prevalent types in 183 HPV positive cases.</p><p><b>CONCLUSION</b>The most prevalent high-risk types of HPV are HPV16 and HPV58 in Huzhou area of Zhejiang province.</p>
Subject(s)
Adolescent , Adult , Female , Humans , Middle Aged , Young Adult , Alphapapillomavirus , Classification , China , Human papillomavirus 16 , Papillomavirus Infections , Virology , Uterine Cervicitis , VirologyABSTRACT
<p><b>OBJECTIVE</b>To investigate the immunological function of a yeast expression system for thymosin alpha1 (Talpha1).</p><p><b>METHODS</b>A constructed Talpha1 yeast expression system was used to investigate the immunological function of orally administered Talpha1. Dried yeast containing three different concentration of Talpha1 was fed to normal Balb/c mice and other Balb/c mice whose immunities were inhibited in advance by cyclophosphamide. Synthesized Talpha1 peptide was used as positive control and dried yeast with empty plasmid was used as negative control. CD4(+) and CD8(+) levels were detected by flow cytometry assay. TNF-alpha, IFN-gamma, IL-2, IL-6 and IL-10 levels were detected by liquid chip.</p><p><b>RESULTS</b>In normal Balb/c mice or immune inhibition Balb/c mice, CD8(+) levels were significantly increased. Especially in immune inhibition Balb/c mice, CD8(+) levels in synthesized Talpha1 group (18.77%+/-4.72%), small dose group (13.48%+/-6.17%) and large dose group (22.74%+/-1.09%) were significantly higher than that in empty yeast control group (7.49%+/-2.14%).</p><p><b>CONCLUSION</b>Orally administered Talpha1 has its certain immunomodulatory function.</p>